In this study, antioxidant, oxidant, antimicrobial activity and heavy metal contents of Morchella esculenta (L.) Pers. mushroom collected from Konya (Turkey) province were determined. Antioxidant and oxidant values were determined by using Rel Assay TAS and TOS kits. Element contents were determined by AAS (atomic absorption spectroscopy). Antimicrobial activities were determined by agar dilution method against bacteria and fungus strains. As a result of the study, TAS (total antioxidant status) value of mushroom was determined as 4.580±0.114 mmoL/L, TOS (total oxidant status) value was determined as 13.549±0.211 μmoL/L and OSI (oxidative stress index) value was found to be 0.296±0.003. Mushroom extracts were found to be effective at concentrations 50-200 μg/mL against the test microorganisms. In addition, the contents of Fe, Cu, Zn, Pb, Ni, Mn, Co, Cd and Cr were determined as 264.57±17.27, 14.77±0.14, 7.82±0.34, 12.59±0.89, 0.53±0.08, 19.45±0.97, 3.82±0.13, 2.45±0.02 and 6.86±0.08 mg.kg-1, respectively. It was determined that M. esculenta can be one of the antioxidant and antimicrobial sources which can be taken by diet.