Effects of different cytoplasmic chaperones on outer membrane solubility in Escherichia coli; rOMP25 modeling
1Department of Molecular Biology and Genetics, Faculty of Arts and Science, Yildiz Technical Universit, Istanbul, Türkiye
Sigma J Eng Nat Sci 2023; 41(5): 907-915 DOI: 10.14744/sigma.2022.00106
Abstract
In this study, for the first time in the literature, is investigated the effects of different chaper-ones on outer transmembrane membrane protein solubility by modeling the expression of the recombinant Omp25 outer membrane protein, which is one of the main antigens against brucellosis and known to form inclusion bodies in the cytoplasm.
Keywords: rOmp25; Brucellosis; Inclusion Body; Molecular Chaperones; Protein Expression; Purification
