Lake Seyfe is a shallow lake 30 km east of the city center of Kırşehir with an average surface area of 34 km2 and located 1110 m above sea level. Due to various interventions (via drainage and/or irrigation channels, groundwater withdrawal with wells, agricultural activities, domestic wastes, etc.) in Seyfe Lake and its surroundings from 1960s to the present, natural functions of the lake have been deteriorated. As a result of the aforementioned actions and changes such as water elevation, shrinking lake surface area, decrease in volume and changes in surface temperature were observed in the lake. In this paper, the seasonal changes in the surface temperature distribution of Seyfe Lake were analyzed with the Geographical Information Systems (GIS) using multi-band Landsat satellite images from various years. In September 1990, the lake surface temperature varied between 10.94-24.14 °C while it varied between 12.36-26.67 °C in May 1990. In May 2020, the temperatures varied between 16.31-34.06 °C, and in September 2020, it varied between 25.67-29.67 °C. When the lake surface temperature distribution was analyzed; it was determined that lower temperatures were observed in the northern parts, which are usually the deepest parts of the lake, while the temperature increased towards the central and coastal parts of the lake. However, it was seen that lake surface temperature has been affected by both climatic conditions and anthropogenic activities from the past to the present.