Plants have been used by many communities for different purposes such as food, shelter, medicine. The present study aimed to determine phenolic content and biological potential of Glaucium alakirensis Aykurt, K. Yıldız & A. Özçandır, which is endemic to Antalya (Turkey). Phenolic composition of the plant was analyzed with HPLC device, while antiradical activity was determined by DPPH method. Antimicrobial tests were conducted with agar dilution method against standard bacteria and fungus strains. Total antioxidant and oxidant status were determined using Rel Assay kits. As a result of HPLC analysis, the presence of catechin, chlorogenic acid, hydroxybenzoic acid, quercetin and gallic acid was determined. Plant ex-tracts were effective against microorganisms at concentrations of 50-200 μg/mL. It was de-termined that the plant has high antiradical activity. It is also thought to be used as a natural source for relieving oxidative stress.