The intestinal microbiota is a set of microorganisms in equilibrium, which naturally shelter the digestive tract, and fulfill several functions beneficial to the host. However, this equilibrium can be influenced by many factors such as the type of diet.For instance, the choice of feeding, whether it’s breast feeding or the use of commercial infant formula, profoundly influences the selection and establishment of microbial strains in the infant’s digestive system. In particular, this microbial strain selection can also contribute to the emergence of antibiotic resistance, posing a significant public health issue and consequently limiting therapeutic options. In this work, we considered it essential to examine the profile of the antibiotic resistance of five (05) bacterial strains isolated from the stools of children fed with commercial milk aged 2 years (02) and residing in the Mascara province (Algeria), against sexes (6) antibiotics commonly used in pediatrics. The results obtained give us evidence that the isolated strains show a marked resistance towards almost all antibiotics. In addition, we noted that 88,88% of isolated strains are hyper-productive of extended-spectrum β-lactamase. Whereas, cephalosporinase production was detected only in (E.faecium) and that the combination erythromycin and gentamicin revealed a phenotype of antagonism.