This study focused on developing an eco-friendly dishwashing liquid to address the environmental and health risks posed by conventional cleaners containing toxic chemicals like phosphates and synthetic additives. The significance lies in reducing water pollution, skin irritation, and ecological harm while maintaining cleaning efficacy. The formulation integrated plant-based surfactants, natural enzymes, and citric acid (pH adjuster), excluding phosphates, chlorine, and artificial compounds. Methods included iterative testing of ingredient ratios, pH optimization (target: 7–9), and performance evaluations via oil-removal tests (ASTM-inspired protocols) and FTIR spectroscopy for compositional verification. Key results demonstrated a pH of 9 (achieved with 7 g/L citric acid), acid value of 1.92 mg KOH/g, and 95% grease removal efficiency—outperforming traditional soap solutions (75% efficiency). The product showed rapid biodegradability (>90% in 28 days) and compliance with EU Ecolabel toxicity thresholds. These findings indicate that eco-friendly formulations can rival conventional detergents in performance while minimizing environmental impact. The work advances prior efforts by uniquely combining pH-adjusted citric acid stabilization with plant-derived surfactants, ensuring both safety and efficacy. Novel use of FTIR spectroscopy provided a rapid quality-control tool, enhancing manufacturing consistency. Quantitative benchmarks (e.g., 95% cleaning efficiency, pH 9) and compliance with international standards position this formulation as a scalable, market-ready alternative. Future work could optimize cold-water performance or integrate antimicrobial natural extracts, broadening applications. This study bridges the gap between eco-conscious design and practical utility, offering a blueprint for sustainable household products.