In this study, roughness measurement verification using acquired images from the cylinder liner surfaces was conducted using Fast Fourier Transform (FFT). Cylinder liners that worked at specific times were used in the measurements. The surface images of the cylinder liners from two different tractor models were captured and inspected by a rotating (Nipkow disc) confocal microscope. The captured images were then preprocessed using high pass filter in order to enhance the image before further image analyses conducted. This study indicated that determination of surface roughness is possible using the image analysis techniques. 2 dimensional (2D) FFT image transformation techniques will be used in the near future extensively to determine the surface characterization of cylinder liners of engines used in tractors over time. In all cylinder liners used this study, the smoother appearance of the lower-middle parts suggested that the piston worked mostly in this region.