2Muğla Sıtkı Koçman University, Faculty of Sciences, Department of Chemistry, MUĞLA
3Yildiz Technical University, Faculty of Mechanical Engineering, Mechanical Engineering Department, Beşiktaş-ISTANBUL
Abstract
Chlorella protothecoides is a precious lipid source that can be used as a biodiesel feedstock. Calcium, carbon, iron, hydrogen, magnesium, nitrogen, oxygen, phosphorus, potassium and sulfur are among the various
elements have to be supplied for the growth of microalgae. Especially, nitrogen and phosphorus have significance in handling the growth ratio and lipid production of microalgae. In this study, the effect of
different mediums (with/without nitrogen) on cell growth and lipid content of Chlorella protothecoides was investigated. Highest lipid extraction efficiency was obtained by using hexane at ambient temperature. The
maximum lipid content of Chlorella protothecoides fed with the nitrogen free Bold Basal medium was 48% (dry weight percent). The composition of fatty acid methyl esters was influenced by alteration of medium.
