Physiological, genetic and transcriptional characterization of Fusarium graminearum isolates
1Institute of Graduate Studies in Sciences, Programme of Molecular Biotechnology and Genetics, Istanbul University, Istanbul, TURKEY
2Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Yeni Yuzyil University, Istanbul, TURKEY
3Department of Molecular Biology and Genetics, Faculty of Sciences, Istanbul University, Istanbul, TURKEY
2Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Yeni Yuzyil University, Istanbul, TURKEY
3Department of Molecular Biology and Genetics, Faculty of Sciences, Istanbul University, Istanbul, TURKEY
Sigma J Eng Nat Sci 2024; 42(2): 450-458 DOI: 10.14744/sigma.2022.00047
Abstract
Fusarium graminearum is the primary cause of Fusarium head blight (FHB) epidemics world-wide. The characterization of F. graminearum isolates via physiological, genetic, and transcrip-tional analysis was aimed in the study. A total of 31 isolates and a reference strain were grown on potato dextrose agar (PDA) for 7 days. According to measurements on the 4th and 7th day of cultivation, their minimum and maximum mean linear growth rates (LGRs) were calculated as 9.62±0.44 to 13.32±0.69 mm/day, respectively. Isolates were grouped as x<10 mm/day and 10
Keywords: Fusarium Graminearum; Linear Growth Rate; Gene Expression; PCR-ITS-RFLP; qRT-PCR
