In the use of solid wood and wood-based composite materials in wooden structures, metal elements such as nails, screws and bolts are used as fasteners. The strength of the connection points depends on many factors. In this study, it was aimed to determine effects of wood species, nail size, grain direction and layer numbers on lateral nail strength of structural plywood panels. Scots pine, black pine and spruce were used as wood species for structural plywood production. Five and seven-ply plywood panels, 10 mm and 14 mm thick, were manufactured by using phenol formaldehyde glue resin. Lateral nail strength test was performed according to ASTM D1761. The specimens were oriented so that the load was applied parallel and perpendicular to the grain of the main axis of plywood panel during the test. Also, nail size was chosen as 6d and 8d for test. As a result of the study, it was seen that the Scots pine plywood gave the highest lateral nail strength values among other wood species. Lateral nail strength values of seven-ply plywood was found higher than five-ply plywood. Lateral nail strength value of the samples using 8d nails was found to be higher than those using 6d nails. Also, it was determined the lateral nail strength values in perpendicular to grain were higher than those in parallel to grain.